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PMC 3684285

doi: 10.1016/j.molmed.2021.06.002 202 ErcoliGFernandesVEChungWYWanfordJJThomsonSBaylissCDet al

Following the reverse transcription, qPCR was performed to quantify the RNA levels of NLRP3, ASC and caspase-1 using SYBR-Green Supermix (Bio-Rad, Hercules, CA, USA) and the data were analyzed using the 2Ct method

In subcutaneous application research, occasional local reactions at the injection site were observed, including mild redness and transient swelling that typically resolved within 24 hours

Keene and Appelbaum 2019

Checking labels ensures that individuals do not unintentionally exceed the dosage guidelines, reducing the toxicity risk